An array of antibody-based immunoassays require the use of secondary reagents that detect mouse, rabbit, rat, or goat primary antibodies. These secondary reagents are usually conjugated to a detection reagent, such as horse radish peroxidase (HRP). HRP is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or the release of light as a byproduct of the reaction. HRP functions optimally at a near-neutral pH and can be inhibited by cyanides, sulfides and azides. In addition, its high turnover rate, good stability, low cost and wide availability of substrates makes HRP the enzyme of choice for most applications. Mouse anti-Rabbit Ig Light-Chain Specific:HRP can be used for chemiluminescent readout in western blot, colorimetric readout in ELISA, and precipitation reactions useful in immunocytochemistry and immunohistochemistry. Mouse anti-Rabbit Ig Light-Chain Specific unconjugated is also available for ELISA sandwich assays and for antibody blocking experiments where no detection reagent is required.

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