Cell surface adhesion protein CD44 is a ubiquitously expressed type I transmembrane protein that has important functions related to cell-cell adhesion and extracellular matrix interactions. The transmembrane protein is post-translationally modified at multiple sites by glycosylation and phosphorylation. CD44 ligands include hyaluronic acid, collagens, laminins, osteopontin, serglycin, and fibronectin. CD44 has been implicated in inflammatory cell functions as well as in tumor growth and metastasis. CD44 is overexpressed in many types of cancer; the interaction between CD44 and HER2 has been linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin to link the actin cytoskeleton to the plasma membrane and the extracellular matrix. These interactions are critical for CD44 function in cell-cell adhesion and cell motility.
References
Cichy, J., et al. (2003) J Cell Biol. 161(5):839 Goodinson, S., et al. (1999) Mol Path. 52(4):189Tsukita, S., et al. (1994) J Cell Biol. 126(2):391
Western blot analysis of human A549 (lanes 1 & 2) and human MDA-MB-231 (lanes 3 & 4) whole cell lysates under native (lanes 1 & 3) and denatured (lanes 2 & 4) conditions. The blots were probed with mouse monoclonal anti-CD44 (CM010) at 1:1000 (lanes 1-4).
Immunocytochemical labeling of CD44 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M010). The antibody was detected using goat anti-mouse DyLight® 594.
*For more information, see UniProt Accession P16070
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
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