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当前位置: 首页 > 产品中心 > Protein_purification_kit > ECM BIOSCIENCES/CD44(透明质酸结合区)M591抗体/100μl/CM5911
商品详细ECM BIOSCIENCES/CD44(透明质酸结合区)M591抗体/100μl/CM5911
ECM BIOSCIENCES/CD44(透明质酸结合区)M591抗体/100μl/CM5911
ECM BIOSCIENCES/CD44(透明质酸结合区)M591抗体/100μl/CM5911
商品编号: CM5911
品牌: ecmbio
市场价: ¥6500.00
美元价: 3250.00
产地: 美国(厂家直采)
公司:
产品分类: 蛋白纯化试剂盒
公司分类: Protein_purification_kit
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Cell surface adhesion protein CD44 is a ubiquitously expressed type I transmembrane protein that has important functions related to cell-cell adhesion and extracellular matrix interactions. The transmembrane protein is post-translationally modified at multiple sites by glycosylation and phosphorylation. CD44 ligands include hyaluronic acid, collagens, laminins, osteopontin, serglycin, and fibronectin. CD44 has been implicated in inflammatory cell functions as well as in tumor growth and metastasis. CD44 is overexpressed in many types of cancer; the interaction between CD44 and HER2 has been linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin to link the actin cytoskeleton to the plasma membrane and the extracellular matrix. These interactions are critical for CD44 function in cell-cell adhesion and cell motility.

 

References
Cichy, J., et al. (2003) J Cell Biol. 161(5):839 Goodinson, S., et al. (1999) Mol Path. 52(4):189Tsukita, S., et al. (1994) J Cell Biol. 126(2):391

Western blot analysis of denatured (lanes 1 & 3) and native (lanes 2 & 4)human MDA-MB-231 whole cell lysates. The blots were probed with mouse monoclonal anti-CD44 (CM5881) at 1:1000 (lanes 1 & 2) or mouse monoclonal anti-CD44 (CM5911) at 1:1000 (lanes 3 & 4).

Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MCF7 cells. The cells were labeled with mouse monoclonal anti-CD44 (clone M591). The antibody was detected using goat anti-mouse DyLight® 594.

Clone M591 was generated from a proprietary antigen related to the hyaluron binding region of human CD44 from the MDA-MB-231 breast cancer cell line.
*For more information, see UniProt Accession P16070
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

Clone M591 detects 80-130 kDa* bands corresponding to the molecular mass of CD44 on SDS-PAGE immunoblots of denatured and native MDA-MB-231 cell lysates. The antibody also detects the denatured and native form of a recombinant human CD44 protein that includes only the hyaluron binding region. Clone M591 can be used in multiple applications including western blot, immunocytochemical labeling, ELISA, and immunoprecipitation, as well as for detecting CD44 in live, unfixed cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY

品牌介绍
ECM Biosciences是一家著名的生物公司,开发新的生物技术的生命科学研究。公司工程与研究机构全球发展创新的工具,以提高研究和治疗相关的神经病理学,癌症,免疫功能障碍和心血管疾病。产品列表:品牌名称1FM0141100微升ECM生物科学FXYD5 / Dysadherin(细胞外区域)小鼠单克隆IgG13AM2091100ulECM生物科学c-Abl(Tyr-245),磷酸化特异性兔多克隆5AP1271100ulECM生物科学Abp1(C端区域)小鼠单克隆IgG17AP1671100ulECM生物科学β-Actin小鼠单克隆IgG2b 9AM2021100ulECM生物科学α-肌动蛋白(C端区域)小鼠单克隆IgG111AP4241100ulECM生物科学β-Adaptin(N端区域)小鼠单克隆IgG113AX125550微克ECM生物科学肌动蛋白(N末端区域)封闭肽15AX167550微克<span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">