Tetraspanins comprise a large superfamily of cell surface-associated membrane proteins with four transmembrane domains. On cell membranes, tetraspanins form networks of various proteins called tetraspanin-enriched microdomains (TEMs). CD63 was the first characterized tetraspanin and it is found in TEMs, as well as late endosomes and lysosomes. In late endosomes, CD63 is enriched on the intraluminal vesicles, and can be secreted as exosomes through fusion of endosomes with the plasma membrane. The complex localization pattern of CD63 suggests that its intracellular trafficking and distribution must be tightly regulated. CD63 contains N-link glycosylation sites that produce diverse CD63 molecules that range from 30 to 60 kDa depending on cell type. CD63 is an important exosomal marker in cancer cells, and may be involved in cancer progression and metastasis.
References
Pols, MS & Klumperman, J (2009) Exp Cell Res. 315(9):1584. Metzelaar, M.J. et al. (1991) J Biol Chem. 266(5):3239.Azorsa, D.O. et al. (1991) Blood. 78(2):280.
Western blot of native human cell lyastes: MeWo (lane 1), and MeWo treated with PNGase F (lane 2), and A549 (lane 3), and A549 treated with PNGase F (lane 4). The blot was probed with mouse monoclonal CD63 (CM0401) at 1:100.
Immunocytochemical labeling of CD63 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal CD63 (CM0401). The antibody was detected using goat anti-mouse Ig DyLight® 594.
*For more information, see UniProt Accession P08962
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
This kit contains:
