Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 (Tyr-835 in E-cadherin) can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.
References
Western blot image of human A431 cells that were probed with rabbit polyclonal anti-E-Cadherin (a.a. 774-786) at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000 (lane 3) or mouse monoclonal anti-E-cadherin (Cytoplasmic) at 1:250 (lane 4) and 1:1000 (lane 5).
Formalin fixed, citric acid treated parafin sections of embryonic Rat E16 intestines. Sections were probed with anti-E-Cadherin (CM1681) then anti-mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King"s College London).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
Product References:
CP1921 Gu, H. et al. (2014) FASEB Journal 28(10):4223. WB: human small airway epithelial cellsCP1921 SalaheldeenE. et al. (2014) J Histo Cytochem. 62(9):632. WB: seminiferous tubulesCP1951 Wang, M. et al. (2014) Cell Mol Neurobiol. 34(1):123 WB: MN9D neuronsCM1681 Milara, J. et al. (2013) Thorax. 68(5):410-20. IF: human pulmonary tissueCM1681 Vittal, R. et al. (2013) AJP Lung Cell Mol Phys 304(6):401. WB: Rat & Human Epithelial CellsRS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblastsMS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western BlotRS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western BlotThis kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
CM1681 | E-Cadherin (Cytoplasmic)Mouse mAb | 50 μl | WB, E, IP, ICC, IHC | Hu, Rt, Ms | 120 |
CP1921 | E-Cadherin (a.a. 774-786) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 120 |
CP1901 | N-Cadherin (a.a. 853-864)[E-Cadherin (a.a. 828-839)] Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 130 |
CP1951 | N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 130 |
MS3001 | Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms | |
RS3251 | Anti-Rabbit Ig Light Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
KIT SUMMARY
The E-cadherin phospho-regulation antibody sampler kit can be used to examine phosphorylation of E-cadherin at Tyr-835. The kit includes monoclonal and polyclonal antibodies to monitor the total level of expression for E-Cadherin and secondary reagents for detection of these antibodies.
