Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.
References
Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). The blots were probed with anti-N-Cadherin (Cytoplasmic) (lanes 1 & 2) and anti-N-cadherin (Tyr-820) (lanes 3-6). The latter antibody was used in the presence of no peptide (lane 4), phospho-N-cadherin (Tyr-820) peptide (lane 5), or phospho-N-cadherin (Tyr-860) peptide (lane 6).
Immunocytochemical labeling of phosphorylated N-Cadherin in pervanadate-treated mouse C2C12. The cells were probed with N-Cadherin (Cytoplasmic) and N-Cadherin(Tyr-860) antibodies, followed by immunofluorescent detection.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
Product References:
CP1751 Chang, Y.J. et al. (2014) Mol Cell Biol. 34(6):1003 WB: PC12-SH2B1� cellCP1951 Wang, M. et al. (2014) Cell Mol Neurobiol. 34(1):123 WB: MN9D neuronsCP1751 Sato, F. et al. (2012) Intern J Molec Med 30:495. WB: Human pancreatic cancer BxPC-3 cellsCP1751 Wu, Y. et al. (2012) Int J Oncol. 41(4):1337. WB: human pancreatic cancer cells (PANC-1 PaCa-2)CM1701 Wang, T.C. et al. (2011) J. Cell. Physiol. 226:2063. WB, ICC: rat PC12 cellsCP1751 Ferreri, D.M. et al. (2008) Cell Comm Adh. 15(4):333. WB: human, bovine VECsThis kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
CM1701 | N-Cadherin (Cytoplasmic) Mouse mAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms | 130 |
CP1751 | N-Cadherin (a.a. 811-824) Rabbit pAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms | 130 |
CP1801 | N-Cadherin (Tyr-820), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 130 |
CP1851 | Unphosphorylated N-Cadherin (Tyr-820) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 130 |
CP1901 | N-Cadherin (a.a. 853-864)[E-Cadherin (a.a. 828-839)] Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 130 |
CP1951 | N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 130 |
KIT SUMMARY
The N-cadherin phospho-regulation antibody sampler kit can be used to examine phosphorylation of N-cadherin at Tyr-820 and Tyr-860. The kit also includes monoclonal and polyclonal antibodies to monitor total expression levels of N-Cadherin.
