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当前位置: 首页 > 产品中心 > Crosslinkers > ECM BIOSCIENCES/A431 Calyculin A Control/100μl/AL9001
商品详细ECM BIOSCIENCES/A431 Calyculin A Control/100μl/AL9001
ECM BIOSCIENCES/A431 Calyculin A Control/100μl/AL9001
ECM BIOSCIENCES/A431 Calyculin A Control/100μl/AL9001
商品编号: AL9001
品牌: ecmbio
市场价: ¥1500.00
美元价: 750.00
产地: 美国(厂家直采)
公司:
产品分类: 蛋白交联剂
公司分类: Crosslinkers
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Calyculin A is a serine/threonine phosphatase inhibitor that inhibits the activity of protein phosphatases PP1 and PP2A. Human carcinoma A431 cells treated with calyculin A for 30 minutes can undergo significant threonine phosphorylation, as shown by western blotting using anti-Phospho-Akt (Thr-34), cat.# AP1001, as compared to untreated, control cell lysates.

Confluent cultures of A431 cells were serum starved overnight. Cells were then either left untreated (Cat.# AL9001) or treated with Calyculin A at a final concentration of 100 nM for 30 minutes at 37°C (Cat. #AL9101). Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 1 mM sodium fluoride, 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

Western blot analysis of A431 cells (20 µg/lane) serum starved overnight (lanes 1 & 3) and Calyculin A (100 nM) treated for 30 minutes (lanes 2 & 4). The blot was probed with anti-phospho-Akt (Thr-34) (lanes 1 & 2) or anti-Akt (lanes 3 & 4).

Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY

品牌介绍
ECM Biosciences是一家著名的生物公司,开发新的生物技术的生命科学研究。公司工程与研究机构全球发展创新的工具,以提高研究和治疗相关的神经病理学,癌症,免疫功能障碍和心血管疾病。产品列表:品牌名称1FM0141100微升ECM生物科学FXYD5 / Dysadherin(细胞外区域)小鼠单克隆IgG13AM2091100ulECM生物科学c-Abl(Tyr-245),磷酸化特异性兔多克隆5AP1271100ulECM生物科学Abp1(C端区域)小鼠单克隆IgG17AP1671100ulECM生物科学β-Actin小鼠单克隆IgG2b 9AM2021100ulECM生物科学α-肌动蛋白(C端区域)小鼠单克隆IgG111AP4241100ulECM生物科学β-Adaptin(N端区域)小鼠单克隆IgG113AX125550微克ECM生物科学肌动蛋白(N末端区域)封闭肽15AX167550微克<span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">