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当前位置: 首页 > 产品中心 > albumin > ECM生物科学/磷酸化-c-Abl(Tyr-412)肽/50μg/AX1275
商品详细ECM生物科学/磷酸化-c-Abl(Tyr-412)肽/50μg/AX1275
ECM生物科学/磷酸化-c-Abl(Tyr-412)肽/50μg/AX1275
ECM生物科学/磷酸化-c-Abl(Tyr-412)肽/50μg/AX1275
商品编号: AX1275
品牌: ecmbio
市场价: ¥1100.00
美元价: 550.00
产地: 美国(厂家直采)
公司:
产品分类: 白蛋白
公司分类: albumin
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is widely expressed and is distributed in both the nucleus and the cytoplasm of cells. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion, and stress response. A variety of stimuli activate c-Abl kinase including integrin activation, PDGF stimulation, and binding to proteins, such as c-Jun. Tyrosine phosphorylation is important for the regulation of c-Abl kinase activity. Tyrosine 245 is located in the linker region between the SH2 and catalytic domains. Phosphorylation of Tyr-245 is involved in activation of c-Abl kinase activity. Tyrosine 412 is located in the kinase activation loop of c-Abl, and phosphorylation of this residue is required for kinase activity. Thus, phosphorylation of Tyr-245 and Tyr-412 may be critical for activation of c-Abl in a variety of cell signaling pathways.

 

References
Pluk, H. et al. (2002) Cell 108:247. Brasher, B.B. et al. (2000) J. Biol. Chem. 275:35631.Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9:179.

Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes. Blots were probed with anti-c-Abl (lane 1), anti-c-Abl (Tyr-412) (lanes 2-5), or anti-c-Abl (Tyr-245) (lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of c-Abl (Tyr-412) (lane 4 & 8) or c-Abl (Tyr-245) (lane 3 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).

Phospho-c-Abl (Tyr-412) synthetic peptide corresponds to amino acid residues around tyrosine 412 of human c-Abl. This peptide sequence has high homology to the conserved site in rat and mouse c-Abl, as well as in viral Abl and BCR-Abl fusion protein.
*For more information, see UniProt Accession P00519
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-c-Abl (Tyr-412) phospho-specific antibody (AP1271) in ELISA, and has been used to block the reactivity of AP1271 during Western blot. In addition, the peptide is recommended for use in blocking AP1271 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY

品牌介绍
ECM Biosciences是一家著名的生物公司,开发新的生物技术的生命科学研究。公司工程与研究机构全球发展创新的工具,以提高研究和治疗相关的神经病理学,癌症,免疫功能障碍和心血管疾病。产品列表:品牌名称1FM0141100微升ECM生物科学FXYD5 / Dysadherin(细胞外区域)小鼠单克隆IgG13AM2091100ulECM生物科学c-Abl(Tyr-245),磷酸化特异性兔多克隆5AP1271100ulECM生物科学Abp1(C端区域)小鼠单克隆IgG17AP1671100ulECM生物科学β-Actin小鼠单克隆IgG2b 9AM2021100ulECM生物科学α-肌动蛋白(C端区域)小鼠单克隆IgG111AP4241100ulECM生物科学β-Adaptin(N端区域)小鼠单克隆IgG113AX125550微克ECM生物科学肌动蛋白(N末端区域)封闭肽15AX167550微克<span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">