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当前位置: 首页 > 产品中心 > albumin > ECM BIOSCIENCES/phospho-Actin (Tyr-53) Peptide/50 μg/AX1675
商品详细ECM BIOSCIENCES/phospho-Actin (Tyr-53) Peptide/50 μg/AX1675
ECM BIOSCIENCES/phospho-Actin (Tyr-53) Peptide/50 μg/AX1675
ECM BIOSCIENCES/phospho-Actin (Tyr-53) Peptide/50 μg/AX1675
商品编号: AX1675
品牌: ecmbio
市场价: ¥1100.00
美元价: 550.00
产地: 美国(厂家直采)
公司:
产品分类: 白蛋白
公司分类: albumin
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four α isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (β and γ). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organisation into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerizaiton. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.

 

References

Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694. Winder, S.J. et al. (2005) J. Cell Sci. 118:651.Baba, T. et al. (2003) J. Immunol. 170: 3762.Jungbluth, A. et al. (1995) FEBS Let. 375:87.

Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide (AX1655) or phospho-Actin (Tyr-53) peptide (AX1675), respectively (right).

Actin synthetic peptide corresponding to amino acid residues in the N-terminal region of human β actin. This sequence is identical to similar regions in all four α actins, as well as in γ actin, and is well conserved in actins from most eukaryotic species.
*For more information, see UniProt Accession P60709
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by actin (Tyr-53) phospho-specific antibody (AP1671) in ELISA, and has been shown to block the reactivity of AP1671 in Western blot and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY

品牌介绍
ECM Biosciences是一家著名的生物公司,开发新的生物技术的生命科学研究。公司工程与研究机构全球发展创新的工具,以提高研究和治疗相关的神经病理学,癌症,免疫功能障碍和心血管疾病。产品列表:品牌名称1FM0141100微升ECM生物科学FXYD5 / Dysadherin(细胞外区域)小鼠单克隆IgG13AM2091100ulECM生物科学c-Abl(Tyr-245),磷酸化特异性兔多克隆5AP1271100ulECM生物科学Abp1(C端区域)小鼠单克隆IgG17AP1671100ulECM生物科学β-Actin小鼠单克隆IgG2b 9AM2021100ulECM生物科学α-肌动蛋白(C端区域)小鼠单克隆IgG111AP4241100ulECM生物科学β-Adaptin(N端区域)小鼠单克隆IgG113AX125550微克ECM生物科学肌动蛋白(N末端区域)封闭肽15AX167550微克<span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">